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MB Sample ID: SA084803
Local Sample ID: | T5 |
Subject ID: | SU001274 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL6/J |
Age Or Age Range: | 12 weeks old |
Weight Or Weight Range: | 25-30 g |
Gender: | Male |
Animal Animal Supplier: | Jax Laboratories |
Animal Housing: | Conventional |
Animal Light Cycle: | regular 12h light /dark cycles |
Animal Feed: | ad libitum normal chow diet |
Animal Water: | ad libitum |
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Sample Preparation:
Sampleprep ID: | SP001282 |
Sampleprep Summary: | Aliquots of 100 µL serum were taken and a mixture of deuterium-labeled internal standards was added to each aliquot, followed by 3x volume of sample of cold methanol (MeOH). Samples were vortexed for 5 minutes and stored at −20 °C overnight. Cold samples were centrifuged at 14,000g for 10 minutes, and the supernatant was then transferred to a new tube and 3 mL of acidified H2O (pH 3.5) was added to each sample prior to C18 SPE. The methyl formate fractions were collected, dried under nitrogen, and reconstituted in 50 µL MeOH:H2O (1:1, by vol). Samples were transferred to 0.5 mL tubes and centrifuged at 20,000g at 4 °C for 10 minutes. (35ul) of supernatant was transferred to LC–MS/MS vials for analysis using the BERG LC–MS/MS mediator lipidomics platform. |