Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA084829

Local Sample ID:	AF33
Subject ID:	SU001275
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation
Chromatography
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MS

Sample Preparation:

Sampleprep ID:	SP001283
Sampleprep Summary:	20 mg fecal samples were pipetted into centrifuge tubes (1.5 mL) in preparation for extraction. The protein was precipitated with 800 μL of methanol and 10 μL of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The samples were ground at 65 KHz for 90 s and centrifuged at 12000 rpm for 15 min at 4 °C. 200 μL of the supernatant was transferred into a vial for further analysis.

Sampleprep ID:

SP001283

Sampleprep Summary:

20 mg fecal samples were pipetted into centrifuge tubes (1.5 mL) in preparation for extraction. The protein was precipitated with 800 μL of methanol and 10 μL of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The samples were ground at 65 KHz for 90 s and centrifuged at 12000 rpm for 15 min at 4 °C. 200 μL of the supernatant was transferred into a vial for further analysis.