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MB Sample ID: SA085964
| Local Sample ID: | BALF_117 |
| Subject ID: | SU001283 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
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Sample Preparation:
| Sampleprep ID: | SP001291 |
| Sampleprep Summary: | 140uL BAL was aliquoted into a microcentrifuge tube containing 20 uL of internal standards. Samples were vortexed followed by protein precipitation with 560 uL cold methanol, and centrifugation (0 C for 15 mins at 18,000 x g). The supernatant was removed and placed into two autosampler vials (165 uL for Hydrophilic Interaction Liquid Chromatography (HILIC) and 495 uL for C18 analysis). The samples were dried in a centrifugal evaporator at 45 C for 2 hours. The samples for Hydrophilic Interaction Liquid Chromatography HILIC analysis were reconstituted in 30 uL of 95:5 water:acetonitrile. The samples for Reversed phase C18 analysis were reconstituted in 90 uL methanol. Samples were stored at -80C prior to MS analysis. |
| Processing Storage Conditions: | Described in summary |
| Extract Storage: | Described in summary |
