Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA086259

Local Sample ID:	BBCT-5
Subject ID:	SU001287
Subject Type:	Invertebrate
Subject Species:	Bombus impatiens
Taxonomy ID:	132113

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Subject
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Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP001295
Sampleprep Summary:	We then pooled three bee abdomens from each cage, freeze-dried the samples, and homogenized the abdomens to a fine powder at 4° C using a bead mill homogenizer. Next, we extracted 10-12 mg of the powder in a 1.5 mL tube with 100 µL of ice-cold extraction solvent (30:30:20:20 acetonitrile:methanol:water:isopropanol) per 1 mg of tissue. We sonicated the samples for 5 minutes in an ice bath, then vortexed them for 30 min at 4° C. Lastly, we centrifuged the samples at 16,000 x g for 15 min at 4° C.

Sampleprep ID:

SP001295

Sampleprep Summary:

We then pooled three bee abdomens from each cage, freeze-dried the samples, and homogenized the abdomens to a fine powder at 4° C using a bead mill homogenizer. Next, we extracted 10-12 mg of the powder in a 1.5 mL tube with 100 µL of ice-cold extraction solvent (30:30:20:20 acetonitrile:methanol:water:isopropanol) per 1 mg of tissue. We sonicated the samples for 5 minutes in an ice bath, then vortexed them for 30 min at 4° C. Lastly, we centrifuged the samples at 16,000 x g for 15 min at 4° C.