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MB Sample ID: SA086269
Local Sample ID: | QC-7 |
Subject ID: | SU001288 |
Subject Type: | Invertebrate |
Subject Species: | Bombus impatiens |
Taxonomy ID: | 132113 |
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Sample Preparation:
Sampleprep ID: | SP001296 |
Sampleprep Summary: | We then pooled three bee abdomens from each cage, freeze-dried the samples, and homogenized the abdomens to a fine powder at 4° C using a bead mill homogenizer. Next, we extracted 10-12 mg of the powder in a 1.5 mL tube with 100 µL of ice-cold extraction solvent (30:30:20:20 acetonitrile:methanol:water:isopropanol) per 1 mg of tissue. We sonicated the samples for 5 minutes in an ice bath, then vortexed them for 30 min at 4° C. Lastly, we centrifuged the samples at 16,000 x g for 15 min at 4° C. |