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MB Sample ID: SA089896
Local Sample ID: | 258_1 |
Subject ID: | SU001306 |
Subject Type: | Cultured cells |
Subject Species: | Plasmodium falciparum |
Taxonomy ID: | 5833 |
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Sample Preparation:
Sampleprep ID: | SP001314 |
Sampleprep Summary: | Following treatment parasites were pelleted, washed with 1 mL of ice-cold 1X PBS, and extracted using 1 mL of ice-cold 9:1 MeOH:Water, containing the internal standard 13C4,15N1-Aspartate. Supernatants were clarified before drying under nitrogen, followed by resuspension in HPLC grade water containing 1 µM chlorpropamide to 1x10^6 parasites/µL for HPLC-MS analysis. 10 µL was injected on a Thermo Exactive Plus Orbitrap mass spectrometer for HPLC-MS-based targeted metabolomics (modified from Lu W et al 2010). |