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MB Sample ID: SA091413
Local Sample ID: | 7 2e6 Mf |
Subject ID: | SU001326 |
Subject Type: | Invertebrate |
Subject Species: | Brugia malayi |
Taxonomy ID: | 6279 |
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Sample Preparation:
Sampleprep ID: | SP001334 |
Sampleprep Summary: | Metabolite extraction – The mass of the weighed worm samples was used to scale the metabolite extraction to a ratio of 16.5 mg / 1 mL extraction solvent. Freezing 80% acetonitrile was added directly to each vial containing the samples, along with zirconium disruption beads (0.5 mm, RPI) and homogenized for 3 min at 4°C in a BeadBlasterTM with a 30 sec on, 30 sec off pattern. The resulting lysate was centrifuged at 21,000 x g for 3 min, and 90% of the supernatant volume was transferred to a 1.5 mL microfuge tube for speed vacuum concentration, no heating. The dry extracts were resolublized in a volume of LCMS grade water 1/10th of that used for the homogenization step, sonicated in a water bath for 3 min, and transferred to a glass insert for analysis. |