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MB Sample ID: SA092047
| Local Sample ID: | CB_11 |
| Subject ID: | SU001334 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Cell Strain Details: | INS-1 832/13 cell line |
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Sample Preparation:
| Sampleprep ID: | SP001342 |
| Sampleprep Summary: | The frozen beta-cell samples were put on dry ice with a randomized order. A volume of 400 µL methanol-water (80:20) was added to the cells and vortexed by a multi-tube vortexer for 5 min at 5000 rpm. All contents in the tube were transferred into a pre-labeled MagNaLyzer tube (with 10-15 beads inside). The tubes were put on the bead homogenizer using quick run setting for bacterial cells (2 ml) with speed at 6.30 m/s for 45 sec in 1 cycle. All samples were centrifuged at 16,000 rcf for 5 min at 4°C. Supernatant from individual sample was transferred into a pre-labeled 1.5 ml low-bind Eppendorf tube. All the samples were centrifuged at 16,000 rcf for 20 min at 4°C. For quality control purpose, 32 µl of the supernatant from individual sample was transferred into a new 2-ml tube to make a sample pool (SP). Aliquots (300 µl) of supernatant from the studied samples and SP were transferred into the pre-labeled 1.5 ml Low-bind Eppendorf tubes. All samples, including studied samples and SPs, were dried using speed-vac. For immediate analysis, 100 µL of Water-Methanol (95:5) was added to the residue, and then thoroughly mixed on multiple tube vortexer for 10 mins at 5000 rpm. After centrifuge at 4°C, 16000 rcf for 4 min, the supernatant from individual sample was transferred to pre-labeled auto-sampler vial for LC-MS analysis. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
