Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA094054

Local Sample ID:	226727_Ilyonectria_mors-panacis-MS2neg
Subject ID:	SU001367
Subject Type:	Fungi
Subject Species:	Ilyonectria mors-panacis;Ilyonectria rufa;Ilyonectria robusta;Ilyonectria torresensis;Ilyonectria estremocensis;Neonectria obtusispora

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Sample Preparation
Chromatography
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MS

Sample Preparation:

Sampleprep ID:	SP001375
Sampleprep Summary:	Cultures were sampled by taking six 0.5-cm plugs from each plate. Plugs were extracted with 4 mL of ethyl acetate, then dried by centrifugal vacuum evaporation. Dried extracts were reconstituted in 1 mL of 3:1 methanol:water and were filtered through 0.45 um PTFE filters (Chromatographic Specialties, Inc.). Composite quality control samples were made by combining 50 uL from each completed extract.

Sampleprep ID:

SP001375

Sampleprep Summary:

Cultures were sampled by taking six 0.5-cm plugs from each plate. Plugs were extracted with 4 mL of ethyl acetate, then dried by centrifugal vacuum evaporation. Dried extracts were reconstituted in 1 mL of 3:1 methanol:water and were filtered through 0.45 um PTFE filters (Chromatographic Specialties, Inc.). Composite quality control samples were made by combining 50 uL from each completed extract.