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MB Sample ID: SA094107
Local Sample ID: | 233_15 |
Subject ID: | SU001368 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 32-67 |
Gender: | Male and female |
Human Race: | Caucasian (C) and African American (AA) |
Human Ethnicity: | non-hispanic |
Human Inclusion Criteria: | Sepsis: 1) Suspected or confirmed infection; 2) Any two of four criteria of systemic inflammatory response in ED(18); 3) Age ≥ 18; 4) Lactate ≥ 2.0 mmol/L; 5) Enrollment within 2 hours of initiation of quantitative resuscitation protocol. Controls: admitted to the emergency department and had no medical conditions that required chronic administration of medication expected to affect platelet function (aspirin, PGY12 inhibitors, etc) |
Human Exclusion Criteria: | Exclusion criteria were:1) any primary diagnosis other than sepsis; 2) established Do Not Resuscitate status; 3) transferred from another hospital with sepsis therapy already initiated; 4) cardiopulmonary resuscitation (chest compression or defibrillation) prior to enrollment; 5) patient or legal representative unable to understand and sign informed consent. |
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Sample Preparation:
Sampleprep ID: | SP001376 |
Sampleprep Summary: | Methanol chlorform extraction of platelet pellet after 2 freeze-thaw cycles in the presence of methanol. |
Sampleprep Protocol ID: | Platelet Pellet MeOHCHCl3 Extraction |
Sampleprep Protocol Filename: | 233.2.1_-_HAPS_Platelet_Pellet_MeOHCHCl3_Extraction |
Processing Storage Conditions: | On ice |
Extraction Method: | Methanol chloroform extraction |
Extract Enrichment: | Lyophilization |
Extract Storage: | -80℃ |
Sample Resuspension: | 500uL 50mM sodium phosphate buffer in D2O |
Sample Spiking: | 4.99 mM DSS internal standard |