Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA094402

Local Sample ID:	H36-II
Subject ID:	SU001380
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Age Or Age Range:	5-28 months
Gender:	Male
Human Ethnicity:	Hispanic
Human Inclusion Criteria:	(1) boys diagnosed with hypospadias with no comorbidities; (2) children undergoing elective circumcision without a clinical diagnosis; (3) age must be between 0 to 28 months; (4) parents' authorization and sign inform consent
Human Exclusion Criteria:	(1) being unable to understand spoken and written language in Spanish; (2) not being willing to provide authorization for collection of tissue sample during the surgical repair of hypospadias and circumcision procedure

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Sample Preparation:

Sampleprep ID:	SP001388
Sampleprep Summary:	Foreskin samples are cut into small pieces with a scalpel (new) on ice. Weight 50 mg of the foreskin for each sample in 1.5 mL Eppendorf tubes. Sample homogenization and extraction of metabolites is perform adding 1 ml of chloroform/MeOH/H2O (2:5:2) to each sample. Samples are sonicated using a polytron for 15 seconds (3X) in a beaker with ice. Shake samples for 15 minutes in a nutating vortex and centrifuge at 13000 rpm for 10 minutes at 4°C. The supernatant is collected and transferred into 1.5 glass vials for drying. For evaporation, use plastic caps with hole caps. Make a little hole in the center for evaporation to take place. Evaporate samples in SpeedVac until samples are completely dried.

Sampleprep ID:

SP001388

Sampleprep Summary:

Foreskin samples are cut into small pieces with a scalpel (new) on ice. Weight 50 mg of the foreskin for each sample in 1.5 mL Eppendorf tubes. Sample homogenization and extraction of metabolites is perform adding 1 ml of chloroform/MeOH/H2O (2:5:2) to each sample. Samples are sonicated using a polytron for 15 seconds (3X) in a beaker with ice. Shake samples for 15 minutes in a nutating vortex and centrifuge at 13000 rpm for 10 minutes at 4°C. The supernatant is collected and transferred into 1.5 glass vials for drying. For evaporation, use plastic caps with hole caps. Make a little hole in the center for evaporation to take place. Evaporate samples in SpeedVac until samples are completely dried.