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MB Sample ID: SA094637
| Local Sample ID: | 2013-BPS-F |
| Subject ID: | SU001384 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL6J |
| Age Or Age Range: | Embryonic day 12.5 |
| Gender: | Male and female |
| Animal Animal Supplier: | Jackson Labs |
| Animal Housing: | polypropylene cages |
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Sample Preparation:
| Sampleprep ID: | SP001392 |
| Sampleprep Summary: | Freshly isolated mouse placneta tissue was snap frozen in liqud nitrogen and no further preparation. Samples were storred at -52C in lyophilyzer and -20C after weighing. Extracts were dried under nitrogen flow and stored at -20C |
| Processing Method: | Frozen tissues were lyophilized for 24 hrs before weighing. |
| Processing Storage Conditions: | Described in summary |
| Extraction Method: | samples were extracted water, containing 25 μg/ml ribitol, vortex for 20 seconds, sonicated for 15 minutes, incubated at 50C for 1h |
| Extract Enrichment: | Sample tubes were sonicated for 15 minutes at 13000g then collected the supernatantant |
| Extract Storage: | -20℃ |
| Sample Resuspension: | Sample was resuspended in 25 μl of pyridine containing 15 mg/ml methoxyamine hydrochloride |
| Sample Derivatization: | Sample was derivatized with 25 μl MSTFA (N-methyl-N-(trimethyl-silyl)trifluoroacetamide) + 1% TMCS (chlorotrimethylsilane) |
| Sample Spiking: | Ribitol was used in the extraction solvent as an internal standard |
