Return to study ST001353 main page
MB Sample ID: SA098168
Local Sample ID: | CKD014 |
Subject ID: | SU001427 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL6J |
Age Or Age Range: | 18-20 weeks |
Weight Or Weight Range: | 20-30g |
Gender: | Male and female |
Animal Animal Supplier: | Jackson Laboratories |
Animal Housing: | 5/cage |
Animal Light Cycle: | 12h |
Animal Feed: | Ad libitum. Control mice received custom casein-diet. Chronic kidney disease was induced by supplementing casein-based diet with 0.15% adenine |
Animal Water: | Ad libitum |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001435 |
Sampleprep Summary: | Muscles were thawed on ice, weighed, and homogenized with a Teflon-tipped conical pestle with a metal rod (Micro-Tube Sample Pestle with Conical Teflon Tip, fits 1.5ml Tubes, autoclavable at 121°F; Research Products International Corp; 199221; Fisher Scientific). The pestle was rinsed with 2-Propanol, water, and methanol and patted dry with a KimWipe in between samples. The samples were centrifuged to pellet the tissue debris and protein concentrations were quantified on the QuBit. The samples were normalized to 500µg/mL of protein with 5mM Ammonium Acetate in water prior to extraction for a total volume of 100µL. 25µL of sample was aliquoted into a clean tube and extracted with 5µL of Global Metabolomics IS and 200µL of 8:1:1 Acetonitrile:Methanol:Acetone to precipitate proteins. The samples were incubated at 4°C for 30 min and centrifuged at 20,000xg at 4°C for 10min. 200µL of supernatant was transferred to a clean Eppendorf tube and dried under nitrogen gas at 30°C and then reconstituted at 25µL in Global Metabolomics Inj. Std. Mix. |