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MB Sample ID: SA099046
| Local Sample ID: | 2019-M-LD BPA |
| Subject ID: | SU001436 |
| Subject Type: | Mammal |
| Subject Species: | Peromyscus Californicus |
| Taxonomy ID: | 42520 |
| Age Or Age Range: | PND90 |
| Gender: | Male and female |
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Sample Preparation:
| Sampleprep ID: | SP001444 |
| Sampleprep Summary: | Fecal samples were snap frozen in liqud nitrogen and no further preparation |
| Processing Method: | Fecal samples were lyophilized for 24 h before weighing |
| Processing Storage Conditions: | -20℃ |
| Extraction Method: | For LCMS analysis lyophilized fecal sample was extracted with 80% Methanol containg 18 µg/ml of umbelliferon, sonicated and vortexed 20 seconds each, shaken at 140 rpmfor 2 h. For GCMS-polar analysis samples were extracted with water, containing 25 μg/ml ribitol, vortex for 20 seconds, sonicated for 20 seconds, incubated at 50C for 1h. For GCMS-nonpolar samples were extracted with chloroform, containing 10 μl/ml docosanol, vortex for 20 seconds, sonicated for 20 second, incubated at 50C for 1h. |
| Extract Enrichment: | Solution were centrifuged at 3000g for 40 min then extracted 0.5 mL of supernatant for LCMS, 1 mL each of supernatant was collected for GCMS-polar and GCMS-nonpolar analysis. |
| Extract Storage: | -20℃ |
| Sample Resuspension: | For GCMS analysis extracts dried under nitrogen flow and storred at -20C then resuspended in 25 μl of pyridine containing 15 mg/ml methoxyamine hydrochloride |
| Sample Derivatization: | GCMS samples were derivatized with 25 μl MSTFA (N-methyl-N-(trimethyl-silyl)trifluoroacetamide) + 1% TMCS (chlorotrimethylsilane) |
| Sample Spiking: | Umbelliferon, ribitol and docosanol were used as internal standard for LCMS, GCMS-polar and GCMS-nonpolar respectively. |
