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MB Sample ID: SA099290
| Local Sample ID: | Sample_12270 |
| Subject ID: | SU001439 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 21-56 |
| Gender: | Male and female |
| Human Inclusion Criteria: | Cystic Fibrosis, adults |
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Sample Preparation:
| Sampleprep ID: | SP001447 |
| Sampleprep Summary: | The extraction solvent was chilled 1% DTT and spiked with 1.33 µM of both internal standards, linezolid-d3 and levofloxacin-d8. Solvent (150 µL) was added to 50 µL of sputum. The samples were vortexed for 30 seconds, shaken at 4˚C on a shaking platform with moderate agitation for 15 minutes, and centrifuged at 13,200 RCF for 10 minutes at 4˚C. The supernatant was pipetted into amber glass vials and injected directly into the LC-MS. |
| Processing Storage Conditions: | On ice |
| Extract Storage: | On ice |
