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MB Sample ID: SA100603
| Local Sample ID: | 22_1uM_Coa_added |
| Subject ID: | SU001450 |
| Subject Type: | Mammalian Subjects |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | postnatal day 56 |
| Gender: | Male |
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Sample Preparation:
| Sampleprep ID: | SP001458 |
| Sampleprep Summary: | Frozen liver tissue was homegenized in 1000 ul h20:methanol (5:3) mixture5 ml and homegenized, 625 ul chloroform was added,vortexed and spun down at 15,000G. 800 ul of top layer was removed and blown down with nitrogen and reconstituted in 400 l 10mM tetrabutylammonium (TBA)+10mM acetate (pH 5). A further 1:16 dilution was performed for analysis of acetyl-CoA. Coenzyme A (CoA) was measured by standard addition method by diluting with TBA containing CoA standard resulting in 1 uM CoA standard addition. 1 uM CoA standard addition samples are denoted in the subject sample factor section. |
| Sampleprep Protocol Filename: | SP_190815_Acetyl-CoA_modified_Metabolomics_polarextraction.docx |
| Processing Method: | Liquid extraction |
| Processing Storage Conditions: | -80 C |
