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MB Sample ID: SA100620
Local Sample ID: | 42_1uM_Coa_added |
Subject ID: | SU001450 |
Subject Type: | Mammalian Subjects |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | postnatal day 56 |
Gender: | Male |
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Sample Preparation:
Sampleprep ID: | SP001458 |
Sampleprep Summary: | Frozen liver tissue was homegenized in 1000 ul h20:methanol (5:3) mixture5 ml and homegenized, 625 ul chloroform was added,vortexed and spun down at 15,000G. 800 ul of top layer was removed and blown down with nitrogen and reconstituted in 400 l 10mM tetrabutylammonium (TBA)+10mM acetate (pH 5). A further 1:16 dilution was performed for analysis of acetyl-CoA. Coenzyme A (CoA) was measured by standard addition method by diluting with TBA containing CoA standard resulting in 1 uM CoA standard addition. 1 uM CoA standard addition samples are denoted in the subject sample factor section. |
Sampleprep Protocol Filename: | SP_190815_Acetyl-CoA_modified_Metabolomics_polarextraction.docx |
Processing Method: | Liquid extraction |
Processing Storage Conditions: | -80 C |