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MB Sample ID: SA100878
Local Sample ID: | mouse_53 |
Subject ID: | SU001453 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | postnatal day 56 |
Gender: | Male |
Animal Housing: | Cage Type: Innovive, Catalog#M-BTM, San Diego, CA; Bedding Type: cellulose fiber chips; |
Animal Light Cycle: | 12:12 |
Animal Feed: | 22/5 Rodent Diet |
Animal Water: | Aquavive, Catalog#M-WB-300A, INNOVIVE, San Diego, CA |
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Sample Preparation:
Sampleprep ID: | SP001461 |
Sampleprep Summary: | Frozen liver tissue was homegenized in 4.6 ml h20:methanol (5:3) mixture and homegenized, 2.9 ml chloroform was added,vortexed and spun down at 5,000G. Top layer was removed and blown down with nitrogen and reconstituted in 400 l 10mM tetrabutylammonium (TBA)+10mM acetate (pH 5). A further 1:16 dilution was performed for analysis of acetyl-CoA. Coenzyme A (CoA) was measured by standard addition method by diluting with TBA containing CoA standard resulting in 1 uM CoA standard addition. 1 uM CoA standard addition samples are denoted in the subject sample factor section. |
Processing Method: | Liquid extraction |
Processing Storage Conditions: | -80℃ |