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MB Sample ID: SA100883
| Local Sample ID: | mouse_63 |
| Subject ID: | SU001453 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Age Or Age Range: | postnatal day 56 |
| Gender: | Male |
| Animal Housing: | Cage Type: Innovive, Catalog#M-BTM, San Diego, CA; Bedding Type: cellulose fiber chips; |
| Animal Light Cycle: | 12:12 |
| Animal Feed: | 22/5 Rodent Diet |
| Animal Water: | Aquavive, Catalog#M-WB-300A, INNOVIVE, San Diego, CA |
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Sample Preparation:
| Sampleprep ID: | SP001461 |
| Sampleprep Summary: | Frozen liver tissue was homegenized in 4.6 ml h20:methanol (5:3) mixture and homegenized, 2.9 ml chloroform was added,vortexed and spun down at 5,000G. Top layer was removed and blown down with nitrogen and reconstituted in 400 l 10mM tetrabutylammonium (TBA)+10mM acetate (pH 5). A further 1:16 dilution was performed for analysis of acetyl-CoA. Coenzyme A (CoA) was measured by standard addition method by diluting with TBA containing CoA standard resulting in 1 uM CoA standard addition. 1 uM CoA standard addition samples are denoted in the subject sample factor section. |
| Processing Method: | Liquid extraction |
| Processing Storage Conditions: | -80℃ |
