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MB Sample ID: SA113456
Local Sample ID: | Evocrp4_1 |
Subject ID: | SU001468 |
Subject Type: | Bacteria |
Subject Species: | Escherichia coli |
Taxonomy ID: | 562 |
Genotype Strain: | K-12 MG1655 |
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Sample Preparation:
Sampleprep ID: | SP001476 |
Sampleprep Summary: | Samples were extracted from the Wildtype, crp mutant and the five evolved replicate population, grown in a bioreactor aerobically in glucose minimal media with 40 mM MOPS at 37 degrees Celsius and 700 rpm. The metabolites were extracted using the Methanol-Chloroform-Water method. The samples were spiked with an equal volume of 13C-labelled internal standard taken from the same batch at an earlier stage to prevent to account for losses due to metabolite degradation and to enable robust quantification circumventing the ion-suppression effects. |