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MB Sample ID: SA121164
Local Sample ID: | 112 |
Subject ID: | SU001504 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
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Sample Preparation:
Sampleprep ID: | SP001512 |
Sampleprep Summary: | 784 normal pregnancy samples (3 outlier samples were removed) were completely randomized within each cohort (Discovery and Validation - 1) and analyzed in 12 batches across two years. 200 μL plasma was extracted by mixing 800 μL 1:1:1 acetone: acetonitrile: methanol with the internal standard mixture. The extraction mixture was vortexed and mixed for 15 min at 4 C and incubated at -20 C for 2 hours to allow protein precipitation. The supernatant was collected after centrifugation and evaporated to dryness under nitrogen (Biotage Turbovap). The dry extracts were reconstituted with 200 μL 1:1 methanol: water before analysis. |