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MB Sample ID: SA125844
Local Sample ID: | 103 |
Subject ID: | SU001565 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
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Sample Preparation:
Sampleprep ID: | SP001573 |
Sampleprep Summary: | Urine aliquots were prepared and analyzed in a random order as previously described (Contrepois et al., 2015). Briefly, frozen urine samples were thawed on ice and centrifuged at 17,000g for 10 min at 4°C. Supernatants (25 µl) were then diluted 1:4 with 75% acetonitrile and 100% water for HILIC- and RPLC-MS experiments, respectively. Each sample was spiked-in with 15 analytical-grade internal standards (IS). Samples for HILIC-MS experiments were further centrifuged at 21,000g for 10 min at 4°C to precipitate proteins. |