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MB Sample ID: SA126717
Local Sample ID: | 96_Old_Liver_26a |
Subject ID: | SU001581 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6JOlaHsd |
Age Or Age Range: | 6 months (young) and 20-24 months (old) |
Gender: | Male |
Animal Animal Supplier: | Envigo |
Animal Housing: | Animal rooms were maintained at 21°C-24°C and 35%-75% relative humidity |
Animal Light Cycle: | 12/12-h (7am to 7pm) dark-light cycle |
Animal Feed: | Altromin 1324 (chow) |
Animal Water: | Ad lib |
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Sample Preparation:
Sampleprep ID: | SP001589 |
Sampleprep Summary: | To maximize accuracy and to take into account tissue inhomogeneity, metabolites were extracted from 3 separate liver pieces for each mouse, and the average metabolite value of the 3 technical replicates was calculated at analysis. In detail, frozen liver pieces weighting ~30mg were transferred into soft tissue homogenizing CK 14 tubes containing 1.4 mm ceramic beads (Bertin corp.) prefilled with 1 ml of cold (-20°C) metabolites extraction solvent (Methanol: Acetonitrile:H2O, 50:30:20) and kept on ice. Samples were homogenized using precellys 24 tissue homogenizer (3X20sec at 6000 rpm, with a 30sec gap between each of the three cycles, Bertin Technologies) cooled to 2°C. Homogenized extracts were centrifuged in the Precellys tubes at 18,000g for 15 minutes at 4°C, supernatants were collected in microcentrifuge tubes and centrifuged again at 18,000g for 10 minutes at 4°C. The supernatants were transferred to glass HPLC vials and kept at -75 °C prior to LC-MS analysis. |