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MB Sample ID: SA136787
Local Sample ID: | YGR023w-2 |
Subject ID: | SU001689 |
Subject Type: | Yeast |
Subject Species: | Saccharomyces cerevisiae |
Taxonomy ID: | 4932 |
Genotype Strain: | BY4742, YGR023w |
Cell Biosource Or Supplier: | Open Biosystems |
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Sample Preparation:
Sampleprep ID: | SP001695 |
Sampleprep Summary: | Extraction of metabolites was performed by homogenization in 1 mL of cold methanol/H2O (1:1) extraction solution and centrifugated at 167 x g at 4°C for 5 min. Supernatants were collected and evaporated to dryness in a nitrogen stream stream at 50 ºC (RapidVap, Labconco), and derivatized by methoxyamination by adding 50 μl of 20 mg/ml solution of methoxyamine hydrochloride in pyridine followed by incubation at 37°C for 2 hours. Trimethylsilylation was subsequently performed by adding 50 µl of N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA+1% TMCS) and incubated for 1 hour at 65°C. Samples were centrifuged at 13000 rpm for 10 minutes at RT. Supernatants were transferred to glass vials for GC/MS analysis |