Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA151762

Local Sample ID:	Spender49_combi_p
Subject ID:	SU001730
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Cell Strain Details:	CD161+ TCR V 7.2+ MAIT cells were obtained by positive magnetic separation after isolation of Peripheral blood mononuclear cells from male human donors.

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Sample Preparation:

Sampleprep ID:	SP001736
Sampleprep Summary:	Cells were quenched 3 times with 1 ml ice-cold 0.9% sodium chloride. After removing the quenching solution, cells were resuspended in 100 µl ice-cold acetonitrile followed by 100 µl ice-cold Milli-Q water. After vortexing for 1 min, cells were centrifuged (14000 rpm, 4 °C, 10 min). Supernatants were transferred to new tubes. Intracellular metabolites were extracted again with 500 µl Methanol:ACN:Milli-Q water (2:3:1). After centrifugation (14000 rpm, 4 ° C, 10 min) both supernatants were combined, evaporated to dryness and stored at -80 °C until measurement.

Sampleprep ID:

SP001736

Sampleprep Summary:

Cells were quenched 3 times with 1 ml ice-cold 0.9% sodium chloride. After removing the quenching solution, cells were resuspended in 100 µl ice-cold acetonitrile followed by 100 µl ice-cold Milli-Q water. After vortexing for 1 min, cells were centrifuged (14000 rpm, 4 °C, 10 min). Supernatants were transferred to new tubes. Intracellular metabolites were extracted again with 500 µl Methanol:ACN:Milli-Q water (2:3:1). After centrifugation (14000 rpm, 4 ° C, 10 min) both supernatants were combined, evaporated to dryness and stored at -80 °C until measurement.