Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA152083

Local Sample ID:	DNA_Adducts_Sample_1
Subject ID:	SU001738
Subject Type:	Synthetic sample
Subject Species:	-

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Sample Preparation:

Sampleprep ID:	SP001744
Sampleprep Summary:	All DNA adduct standards were purchased or prepared as previously described. The nine DNA adduct standards included: O6-Methyl-2´-deoxyguanosine (O6-me-dG), 8-oxo-7, 8-dihydro-2´-deoxyguanosine (8-oxo-dG), N6-hydroxymethyldeoxyadenosine (N6-Me-dA), 1, N6-etheno-2´-deoxyadenosine (ε-dA), N2-Ethyl-2´-deoxyguanosine (N2-ethyl-dG), (6R/S)-3-(2´-deoxyribos-1´-yl)-5,6,7,8-tetrahydro-6-hydroxypyrimido[1,2-a]-purine-10(3H)one (OH-PdG), O2-[4-(3-pyridyl)-4-oxobut-1-yl]thymidine (O2-POB-dT), D5-ethyl-2´-deoxycytidine (D5-ethyl-dC), 6-(1-Hydroxyhexanyl)-8-hydroxy-1, and N2-propano-2´-deoxyguansine (HNE-dG). The nine standards were dissolved in 20% methanol and combined at a final concentration of 10 fmol/µL, respectively. All solvents were LC-MS grade and were purchased from Sigma-Aldrich.

Sampleprep ID:

SP001744

Sampleprep Summary:

All DNA adduct standards were purchased or prepared as previously described. The nine DNA adduct standards included: O6-Methyl-2´-deoxyguanosine (O6-me-dG), 8-oxo-7, 8-dihydro-2´-deoxyguanosine (8-oxo-dG), N6-hydroxymethyldeoxyadenosine (N6-Me-dA), 1, N6-etheno-2´-deoxyadenosine (ε-dA), N2-Ethyl-2´-deoxyguanosine (N2-ethyl-dG), (6R/S)-3-(2´-deoxyribos-1´-yl)-5,6,7,8-tetrahydro-6-hydroxypyrimido[1,2-a]-purine-10(3H)one (OH-PdG), O2-[4-(3-pyridyl)-4-oxobut-1-yl]thymidine (O2-POB-dT), D5-ethyl-2´-deoxycytidine (D5-ethyl-dC), 6-(1-Hydroxyhexanyl)-8-hydroxy-1, and N2-propano-2´-deoxyguansine (HNE-dG). The nine standards were dissolved in 20% methanol and combined at a final concentration of 10 fmol/µL, respectively. All solvents were LC-MS grade and were purchased from Sigma-Aldrich.