Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA152137

Local Sample ID:	mv08618_28.mzXML
Subject ID:	SU001739
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Pooled

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MS

Sample Preparation:

Sampleprep ID:	SP001745
Sampleprep Summary:	Sample treatment was performed according to a previous study 22. Briefly, after centrifugation, 40 µl of urine cleaned supernatant were transferred to a 96 well-plate for LC-MS (liquid chromatography-mass spectrometry) analysis and diluted by adding 50 µl of H2O (0.1 % v/v HCOOH). Each sample was spiked with 10 µl of 20 µM IS solution containing phenylalanine-d5, caffeine-d9, leukine enkephaline and reserpine in H2O:CH3OH (1:1, 0.1% v/v HCOOH). Blank samples were prepared by replacing urine by ultrapure H2O. A quality control (QC) sample was prepared by mixing 10 µl from each urine sample.

Sampleprep ID:

SP001745

Sampleprep Summary:

Sample treatment was performed according to a previous study 22. Briefly, after centrifugation, 40 µl of urine cleaned supernatant were transferred to a 96 well-plate for LC-MS (liquid chromatography-mass spectrometry) analysis and diluted by adding 50 µl of H2O (0.1 % v/v HCOOH). Each sample was spiked with 10 µl of 20 µM IS solution containing phenylalanine-d5, caffeine-d9, leukine enkephaline and reserpine in H2O:CH3OH (1:1, 0.1% v/v HCOOH). Blank samples were prepared by replacing urine by ultrapure H2O. A quality control (QC) sample was prepared by mixing 10 µl from each urine sample.