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MB Sample ID: SA156946
Local Sample ID: | C-23YH9-BR-00 |
Subject ID: | SU001769 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
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Sample Preparation:
Sampleprep ID: | SP001775 |
Sampleprep Summary: | Prior to sample preparation, breast milk samples were thawed on ice and then vortexed thoroughly to prevent separation of lipid-rich and aqueous phases. Extracts were prepared by treating 75μL of breastmilk sample with 150μL of LC-MS grade acetonitrile containing a series of XX 13C-labelled internal standards (listed below), vortexed, and allowed to equilibrate at 4C for 30 minutes. The extract was then centrifuged for 10 mins at 18,1000 x g and 4C, and 50μL of extract was transferred to an LC autosampler vial containing 50μL of water and placed in a refrigerated autosampler until analysis. Following the same protocol, for each batch matrix blank (replacing the breast milk with H2O) and multiple QAQC samples were extracted. Internal standards used include [13C3]-Cortisol, [13C6]-NNAL, [trimethyl-13C3]-caffeine, [13C8]-PFOA, [13C8]-PFOS, [13C5]-L-methionine, [13C3]-Cotinine, [13C9, 15N]-L-tyrosine, [13C5, 15N]-L-glutamic acid, [13C3]-glyphosate, [13C6]-D-glucose, [13C9]-PFNA, [13C6]-PFHxA, [13C6]-PFHxS, [13C9]-PFDeA and [13C9]-PFUDeA, and were added at levels comparable to those in human plasma samples. |
Processing Storage Conditions: | 4℃ |
Extraction Method: | Protein precipitation with acetontrile. |