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MB Sample ID: SA162132
Local Sample ID: | Plasma_neg_27 |
Subject ID: | SU001799 |
Subject Type: | Mammal |
Subject Species: | Sus scrofa |
Taxonomy ID: | 9823 |
Age Or Age Range: | 0-125 days |
Weight Or Weight Range: | 1-80 kg |
Gender: | Male |
Animal Animal Supplier: | Swine Innovation Centre Sterksel, the Netherlands |
Animal Housing: | Metabolism cages |
Animal Light Cycle: | 12h light / 12h darknes |
Animal Feed: | Experimental diets (see publication) |
Animal Water: | Ad libitum |
Animal Inclusion Criteria: | Birth weight |
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Sample Preparation:
Sampleprep ID: | SP001805 |
Sampleprep Summary: | Plasma samples (150 µL) were pipetted into a 96-well plate and 450 µL acetonitrile containing an internal standard mix of glycocholic acid (glycine-1-13C) and p-chlorophenylalanine to a final concentration of 0.01 mg/mL, was added per well. The samples were mixed immediately and placed at 4°C for 10 min for protein precipitation. After centrifugation (3700 rpm for 25 min at 4°C), the supernatant was transferred to a filter plate fixed on top of a 96-well collection plate in a manifold with a pressure gauge. Vacuum was then applied to the filter plate, and the solvent containing plasma components was collected into the 96-well collection plate. The collection plate was placed in a vacuum centrifuge and the samples were evaporated to dryness (ca. 2.5 h, 805 × g and 30°C). A volume of 150 µL of water/acetonitrile/formic acid (95/5/0.1%) was added to the dried extracts in the collection plate. The dried collection plate was then sealed with a piece of aluminum laminate (#186002789, Waters), centrifuged (3700 rpm for 25 min at 4°C), and kept in the auto-sampler at 10°C for the UPLC-QTOF/MS analysis. The injection volume was 3 µl. |
Processing Storage Conditions: | -80℃ |
Extract Storage: | -80℃ |