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MB Sample ID: SA162351
Local Sample ID: | 6 |
Subject ID: | SU001801 |
Subject Type: | Mammal |
Subject Species: | Sus scrofa |
Taxonomy ID: | 9823 |
Age Or Age Range: | 0-125 days |
Weight Or Weight Range: | 1-80 kg |
Gender: | Male |
Animal Animal Supplier: | Swine Innovation Centre Sterksel, the Netherlands |
Animal Housing: | Metabolism cages |
Animal Light Cycle: | 12h light / 12h darknes |
Animal Feed: | Experimental diets (see publication) |
Animal Water: | Ad libitum |
Animal Inclusion Criteria: | Birth weight |
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Sample Preparation:
Sampleprep ID: | SP001807 |
Sampleprep Summary: | In a 96-well plate, the urine samples (112.5 µL) were diluted with H2O (112.5 µL) and 25 µL acetonitrile (100%, ACN) containing an internal standard mix of glycocholic acid (glycine-1-13C) and p-chlorophenylalanine to a final concentration of 0.01 mg/mL was added. The samples were incubated for 20 min at 4°C and subsequently centrifuged (3700 rpm, 25 min, 4°C). The supernatant, 100 µL per well, was transferred to a 200 µL 96-well plate and a protective film was welded on the plate using a heat sealer, and the plate was centrifuged at 3700 rpm, 4°C for 25 min prior to the LC-MS analysis. Samples were kept in the autosampler at 10°C, and the injection volume was 3 µl. |