Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA162649

Local Sample ID:	POL_19
Subject ID:	SU001810
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP001816
Sampleprep Summary:	Plasma proteins were removed by adding 300 µL of cold (-20 ℃) methanol:ethanol (1:1) to 100 µL of sample. Samples were then vortex-mixed and stored on ice for 5 min. Supernatant containing the metabolites was separated from the pellet by centrifugation (16,000 × g for 20 min at 4 ℃), then put into a LC vial for analysis. Quality control sample (QC) was prepared by pooling equal volumes of plasma from each sample. QC followed the same procedure applied for the experimental samples and was analysed throughout the run to provide a measurement of system stability, performance and reproducibility. All samples were randomised before metabolite extraction and for the corresponding analytical run.

Sampleprep ID:

SP001816

Sampleprep Summary:

Plasma proteins were removed by adding 300 µL of cold (-20 ℃) methanol:ethanol (1:1) to 100 µL of sample. Samples were then vortex-mixed and stored on ice for 5 min. Supernatant containing the metabolites was separated from the pellet by centrifugation (16,000 × g for 20 min at 4 ℃), then put into a LC vial for analysis. Quality control sample (QC) was prepared by pooling equal volumes of plasma from each sample. QC followed the same procedure applied for the experimental samples and was analysed throughout the run to provide a measurement of system stability, performance and reproducibility. All samples were randomised before metabolite extraction and for the corresponding analytical run.