Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA163296

Local Sample ID:	LPS12C3
Subject ID:	SU001821
Subject Type:	Cultured cells
Subject Species:	Rattus norvegicus
Taxonomy ID:	10116

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Sample Preparation:

Sampleprep ID:	SP001827
Sampleprep Summary:	Cells were then washed with phosphate buffer solution and high-performance liquid chromatography (HPLC)-grade water, quenched with 1 mL cold HPLC-grade methanol, scraped from the plate, and pelleted. Pellets were dried on a SpeedVac and subsequently lyophilized. Dried samples were weighed out and extracted with the solvent volumes adjusted to maintain a ratio of 1 mL of solvent per 1 mg of dried cellular material. The final volume of reconstitution solvent was adjusted to 100 μL per 1 mg of dried material. All cell-culture conditions (unlabeled versus labeled, control versus LPSstimulated) were sampled in triplicate.

Sampleprep ID:

SP001827

Sampleprep Summary:

Cells were then washed with phosphate buffer solution and high-performance liquid chromatography (HPLC)-grade water, quenched with 1 mL cold HPLC-grade methanol, scraped from the plate, and pelleted. Pellets were dried on a SpeedVac and subsequently lyophilized. Dried samples were weighed out and extracted with the solvent volumes adjusted to maintain a ratio of 1 mL of solvent per 1 mg of dried cellular material. The final volume of reconstitution solvent was adjusted to 100 μL per 1 mg of dried material. All cell-culture conditions (unlabeled versus labeled, control versus LPSstimulated) were sampled in triplicate.