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MB Sample ID: SA163297
Local Sample ID: | LPS12C2 |
Subject ID: | SU001821 |
Subject Type: | Cultured cells |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
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Sample Preparation:
Sampleprep ID: | SP001827 |
Sampleprep Summary: | Cells were then washed with phosphate buffer solution and high-performance liquid chromatography (HPLC)-grade water, quenched with 1 mL cold HPLC-grade methanol, scraped from the plate, and pelleted. Pellets were dried on a SpeedVac and subsequently lyophilized. Dried samples were weighed out and extracted with the solvent volumes adjusted to maintain a ratio of 1 mL of solvent per 1 mg of dried cellular material. The final volume of reconstitution solvent was adjusted to 100 μL per 1 mg of dried material. All cell-culture conditions (unlabeled versus labeled, control versus LPSstimulated) were sampled in triplicate. |