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MB Sample ID: SA164944
Local Sample ID: | S4R3 |
Subject ID: | SU001853 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Female |
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Sample Preparation:
Sampleprep ID: | SP001859 |
Sampleprep Summary: | cells were washed gently with 150 mM ammonium acetate buffer and scraped quickly with a rubber-tipped cell scraper. Subsequently, 500 uL chilled 80% methanol was added and the scraped cells were sonicated using a bioruptor (Diagenode, Philadelphia, PA, USA) in 4 °C water bath during 10 cycles (30s on and 30s off). After that, the sample was placed at -20 °C for 3 h, followed by 10 min of centrifugation at 14000 g at 4 °C. The supernatant containing the metabolite was dried by speedvac and stored at -80 °C until LC-HRMS analysis.The residual protein pellet was dissolved in protein extraction buffer and quantified for normalization. |
Processing Storage Conditions: | 4℃ |
Extract Storage: | -80℃ |
Sample Resuspension: | Sampe were reconstituted in 50% ACN. The volume was adjusted by the protein concentration. |