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MB Sample ID: SA170144
Local Sample ID: | S2_0089 |
Subject ID: | SU001907 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Sample Preparation:
Sampleprep ID: | SP001913 |
Sampleprep Summary: | Hepatic lipids were extracted from the liver samples according to the modified Bligh and Dyer method. Briefly, 1 mL of extraction solution (methanol:chloroform:water = 5:2:2) containing 100 μM dibutylhydroxytoluene, 100 μM ethylenediaminetetraacetic acid, and 100 nM PC15:0/18:1-d7 was added to a frozen tissue sample (wet weight: approx. 50 mg), and then, the sample was homogenized using a Macro Smash homogenizer. Subsequently, the extraction solutions were sonicated on ice bath for 5 min. After centrifugation (6,000 g, 10 min, 4°C), 700 µL of the supernatant was collected, and then, 235 µL chloroform and 155 µL water were added to the supernatant. The organic layer was collected in a glass tube and dried under a stream of nitrogen gas; the dried residue was dissolved in methanol (200 µL) and stored at −80 °C before performing the LC/HRMS/MS experiments. |