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MB Sample ID: SA171028
| Local Sample ID: | QCpool g1 D_NLN_subcu_081.d |
| Subject ID: | SU001915 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | albino C57BL/6 |
| Age Or Age Range: | 6-8 weeks |
| Gender: | Female |
| Animal Animal Supplier: | Jackson Laboratories (Bar Harbor, ME) |
| Animal Housing: | NCI-Bethesda Animal Facility |
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Sample Preparation:
| Sampleprep ID: | SP001921 |
| Sampleprep Summary: | Serum (50 µL) was transferred to 200 μL ice-chilled (4°C) MilliQ H2O. Tissue (~16 mg) was measured from subcutaneous and intracranial samples followed by addition of 250 μL MilliQ H2O. Then, samples were sonicated at 40 amps (~30 s) until homogeneous. 80 μL of at 0.150 µg/mL debrisoquine in 60% methanol (MeOH)/40% water(aq) reagent was added. 500 μL chilled (-20°C) MeOH was added, vortexed (med) and incubated 15 min on ice. 250 μL chilled (-20°C) Chloroform was added, vortexed (high) and incubated 20 min in ice on rotating mixer. Mixture was centrifuged (13,000x g) for 18 min at 4°C. 705 μL of hydrophilic upper layer was aspirated and transferred to separate 1.5 mL microtubes, dried to completion under N2 gas sample concentrator, and stored at -80°C |
| Sampleprep Protocol ID: | Extraction |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | LCMS Extraction |
| Extract Cleanup: | Bligh-Dyer biphasic separation, discard protein disk and dried to completion under N2 gas |
| Sample Resuspension: | 80 uL 60% MeOH (aq) |
| Sample Spiking: | Internal standard 0.150 µg/mL debrisoquine(IS) was added to each |
