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MB Sample ID: SA171106
| Local Sample ID: | GE-GC-EA(s) |
| Subject ID: | SU001916 |
| Subject Type: | Other organism |
| Subject Species: | Gracilaria edulis |
| Taxonomy ID: | 172966 |
| Gender: | Not applicable |
| Species Group: | Rhodophyta |
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Sample Preparation:
| Sampleprep ID: | SP001922 |
| Sampleprep Summary: | Essentially, for both approaches, the seaweeds were rinsed sequentially with seawater followed by normal tap and then double distilled water to eradicate dirt and impurities. Clean samples were then dried using a freeze-dryer and later crushed into fine granule powder using an electric grinder. Different fractions of extracts were prepared using 10 grams of each powder to dissolve them in 100 mL of the mentioned solvents. All the prepared mixtures were made homogeneous using a rotating shaker (Yihder LM-530D, Shaker, Taiwan) for 24 hours and finally centrifuged (Eppendorf 5810 R Centrifuge, Germany) at 4000 rpm for 10 min at 4◦C to separate the supernatant. Each of the clear supernatants of the extracts was concentrated via a Rotary evaporator (Thermo Fisher Scientific EYELA N-1200A Rotary Evaporator, Tokyo). A further concentration using a vacuum concentrator (LaboGene, Brigachtal, Germany) was done to obtain a viscous liquid for storage at 4◦C and future experiments. |
| Processing Storage Conditions: | Room temperature |
| Extract Storage: | 4℃ |
