Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA171649

Local Sample ID:	840422-27_018
Subject ID:	SU001922
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Age Or Age Range:	23-73
Gender:	Male and female
Human Race:	Hispanic and Caucasian

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Sample Preparation:

Sampleprep ID:	SP001928
Sampleprep Summary:	1 Thaw plasma samples on ice 2 Vortex plasma sample and pipette 50µl in polypropylene Eppendorf tube/Plate 3 Add Surrogate Standards of the 3 lipid classes (as needed) and other reagents as in the following table: Plasma Sample 10x Oxylipid Surrogate STDs CUDA + PHAU Anti-oxidant ACN/MeOH SUM 4 Vortex for 30sec 5 Centrifuge at 6°C for 5 min at 15,000 g (or 1,800 g for plates) 6 Take the supernatant (~240µl) into new Eppendorf tube/filter plate 7 Spin Filter (0.1 um) or plate filter (0.2um) for 5 min @ 12,000 g (1,800g for plate) 8 place aliquotes of samples into vials, or plate, and cap vials or seal plates 9 Store in -20°C until analysis

Sampleprep ID:

SP001928

Sampleprep Summary:

1 Thaw plasma samples on ice 2 Vortex plasma sample and pipette 50µl in polypropylene Eppendorf tube/Plate 3 Add Surrogate Standards of the 3 lipid classes (as needed) and other reagents as in the following table: Plasma Sample 10x Oxylipid Surrogate STDs CUDA + PHAU Anti-oxidant ACN/MeOH SUM 4 Vortex for 30sec 5 Centrifuge at 6°C for 5 min at 15,000 g (or 1,800 g for plates) 6 Take the supernatant (~240µl) into new Eppendorf tube/filter plate 7 Spin Filter (0.1 um) or plate filter (0.2um) for 5 min @ 12,000 g (1,800g for plate) 8 place aliquotes of samples into vials, or plate, and cap vials or seal plates 9 Store in -20°C until analysis