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MB Sample ID: SA173357
Local Sample ID: | Clostridiales St.46-1 (50uM_LCA_pH7) |
Subject ID: | SU001929 |
Subject Type: | Bacteria |
Subject Species: | Bacteria |
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Sample Preparation:
Sampleprep ID: | SP001935 |
Sampleprep Summary: | 100 μL of culture supernatant was transferred into a screw-cap glass vial containing 10 μL of deuterium-labelled internal standards (d4-CA, d4-CDCA, and d4-LCA, 1 nmol/mL each). 400 μL of water was added and sonicated for 10 min, then applied onto the solid-phase extraction cartridge (Agilent Bond Elut C18, 100 mg/1 mL, preconditioned with 1 mL of methanol and 3 mL of water). The cartridge was washed with 1 mL of water and captured bile acids were eluted with 1 mL of 90% ethanol. After solvent evaporation, the remaining residue was dissolved in 100 μL of 50% ethanol |