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MB Sample ID: SA174968
| Local Sample ID: | Control 12 |
| Subject ID: | SU001958 |
| Subject Type: | Other organism |
| Subject Species: | Alexandrium minutum |
| Taxonomy ID: | 39455 |
| Genotype Strain: | CCMP 113 |
| Gender: | Not applicable |
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Sample Preparation:
| Sampleprep ID: | SP001964 |
| Sampleprep Summary: | Non-polar extracts were dissolved at a concentration equivalent to 2.27 x 10^7 A. minutum cells mL-1 in dimethyl sulfoxide d6 (99.9% atom d6-DMSO; Cambridge Isotope Labs) containing 0.1% trimethylsilane (TMS), as internal standard. The media blank extract was prepared in the smallest volume of solvent possible (175 µL). The non-polar extracts were transferred to 3 mm NMR tubes, and analyzed using a Bruker Avance IIIHD 800 MHz NMR spectrometer equipped with a 3 mm triple resonance broadband cryoprobe. Spectra of non-polar extracts were acquired using a simple 1H NMR experiment (Bruker zg30 pulse sequence) with a spectral width of 9.6 kHz centered at 4400.00 Hz over 320 scans. One treatment replicate was lost and therefore its spectrum was not acquired. |
| Sampleprep Protocol Filename: | Predator Cues Target Signaling Pathways in Toxic Algal Metabolome_protocol.pdf |
| Extract Storage: | -80℃ |
