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MB Sample ID: SA175062
| Local Sample ID: | Control 11 |
| Subject ID: | SU001960 |
| Subject Type: | Other organism |
| Subject Species: | Alexandrium minutum |
| Taxonomy ID: | 39455 |
| Genotype Strain: | CCMP 113 |
| Gender: | Not applicable |
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Sample Preparation:
| Sampleprep ID: | SP001966 |
| Sampleprep Summary: | Polar extracts were dissolved at a concentration equivalent to 1.70 x 10^7 A. minutum cells mL-1 in 90:10 H2O/D2O (99.96% atom D2O; Cambridge Isotope Labs) with 0.2 mM phosphate buffer (pH 7.4) and 0.25 mM 3 (trimethylsilyl)propionic 2,2,3,3d 4 acid (TMSP), as internal standard, for 1H NMR spectroscopy analysis. The media blank extract was prepared in the smallest volume of solvent possible (175 µL). Particulates were excluded by briefly centrifuging the samples and then carefully transferring the extracts into 3 mm NMR tubes. The extracts were analyzed using a Bruker Avance IIIHD 800 MHz NMR spectrometer equipped with a 3 mm triple resonance broadband cryoprobe. Spectra for the polar extracts were acquired using a 1D water presaturation (Bruker zgpr pulse sequence) with a spectral width of 12 kHz centered at 3758.36 Hz, with a relaxation delay 3 s, over 480 scans. |
| Sampleprep Protocol Filename: | Predator Cues Target Signaling Pathways in Toxic Algal Metabolome_protocol.pdf |
| Extract Storage: | -80℃ |
