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MB Sample ID: SA175619
Local Sample ID: | 1052308415 |
Subject ID: | SU001967 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP001973 |
Sampleprep Summary: | Plasma samples were thawed on ice, prepared and analyzed randomly as previously described (Contrepois et al., 2015, Contrepois et al., 2018). Briefly, metabolites were extracted using 1:1:1 acetone:acetonitrile:methanol, evaporated to dryness under nitrogen and reconstituted in 1:1 methanol:water before analysis. Fifteen labeled metabolite internal standards (IS) were added to each sample to control for extraction efficiency and LC-MS performance. Complex lipids were prepared using a biphasic separation with cold methyl tert-butyl ether (MTBE), methanol and water and reconstituted in 9:1 methanol:toluene. Each sample was spiked-in with deuterated lipid IS (Sciex, cat#: 5040156) used for quantification. For quality controls, 3 reference plasma samples (40 µl plasma) and 1 preparation blank were processed in parallel. |