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MB Sample ID: SA176007
| Local Sample ID: | Acatenella 8 |
| Subject ID: | SU001973 |
| Subject Type: | Other organism |
| Subject Species: | Alexandrium catenella; Alexandrium tamarense |
| Taxonomy ID: | 2925; 2926 |
| Genotype Strain: | CCMP 1719; CCMP 2023 |
| Gender: | Not applicable |
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Sample Preparation:
| Sampleprep ID: | SP001979 |
| Sampleprep Summary: | Non polar extracts were dissolved with volumes calculated to account for different cell densities at the time of harvesting, to a concentration equivalent to 1.62 x 10^7 Alexandrium cells mL-1 in dimethyl sulfoxide d6 (99.9% atom d6-DMSO; Cambridge Isotope Labs) containing 0.1% trimethylsilane (TMS) as internal standard for 1H nuclear magnetic resonance (NMR) spectroscopy analysis. The extracts were transferred to 3 mm NMR tubes. Extracts from Experiment 1 (A. tamarense-A. catenella) were analyzed using a Bruker Avance IIIHD 800 MHz NMR spectrometer equipped with a 3 mm triple resonance broadband cryoprobe. Spectra of non-polar extracts from both experiments were acquired using a Bruker zg30 pulse sequence 1H NMR experiment compiled from 320 scans. Due to poor NMR spectral quality one A. tamarense replicate spectrum was removed from analysis of Experiment 1 (A. tamarense-A. catenella. |
| Sampleprep Protocol Filename: | Differentiating toxic and nontoxic congeneric harmful algae using the non-polar metabolome_protocol.pdf |
| Extract Storage: | -80℃ |
