Return to study ST001904 main page
MB Sample ID: SA176605
Local Sample ID: | OMV-1526-2 |
Subject ID: | SU001982 |
Subject Type: | Bacteria |
Subject Species: | Bacteroides thetaiotaomicron |
Taxonomy ID: | 818 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001988 |
Sampleprep Summary: | Total lipids from OMV and TM were extracted based on the Bligh and Dyer chloroform:methanol method (1). Briefly, 2 volumes of methanol, 1 volume of chloroform, and 0.8 volumes of Milli-Q water were added to 1 volume of PBS-resuspended OMV or TM fractions into solvent-resistant glass tubes. Contents were mixed for 1 min by vortexing and 1 volume of chloroform was added to the mixture. Contents were mixed for another minute and tubes were centrifuged for 5 min at 4000 rpm. After centrifugation, bottom phase (organic) was recovered using a glass Pasteur pipette and stored in solvent-sealed vials at -80°C until lipid analysis by LC-MS. References: 1. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959;37(8):911-7. |