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MB Sample ID: SA178181
Local Sample ID: | 12 |
Subject ID: | SU002004 |
Subject Type: | Invertebrate |
Subject Species: | Drosophila melanogaster |
Taxonomy ID: | 7227 |
Gender: | Male and female |
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Sample Preparation:
Sampleprep ID: | SP002010 |
Sampleprep Summary: | Metabolites were extracted by homogenizing samples in 200uL HPLC water (Sigma) using a TissueLyser (TissueLyser II, Qiagen) for 6 minutes at 25 Hz at 4°C. We then added 800uL methanol and incubated for 30 minutes on dry ice. The suspension was homogenized again for 10 minutes at 25Hz, then spun at 14,000rcf for 10 minutes at 4°C. The supernatant was transferred to a new tube and dried in a Speed-Vac at 30°C. |