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MB Sample ID: SA183468
| Local Sample ID: | 57 |
| Subject ID: | SU002022 |
| Subject Type: | Other organism |
| Subject Species: | Thalassiosira pseudonana |
| Taxonomy ID: | 296543 |
| Genotype Strain: | CCMP1335 |
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Sample Preparation:
| Sampleprep ID: | SP002028 |
| Sampleprep Summary: | Phytoplankton cells were removed from filters using a sonicator SLPe (Branson) in ultra-pure water (Millipore), concentrated by a lyophilizer (Labconco), and kept -80oC until further processing. The samples were mixed with 600 µL of 30 mmol L-1 sodium phosphate buffer (18 mmol L-1 NaHPO4, 12 mmol L-1, pH 7.4) and an internal standard of 2,2-dimethyl-2-silapentane-5-sulfonate-d6 (DSS, 1 mmol L-1), vortexed at 4oC for 5 minutes, centrifuged at 20,800 rcf using an ultracentrifuge (Eppendorf) at 4oC for 10 minutes, and supernatants were transferred to 5-mm NMR tubes (Bruker). All the sample preparation steps were done on ice or in a cold room (4oC). |
| Sampleprep Protocol Filename: | 4_Sample preparation protocol_UGA_phytoplankton_ Aug2021.docx |
| Sampleprep Protocol Comments: | Details are in 4_Sample preparation protocol_UGA_phytoplankton_ Aug2021.docx. |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Water |
| Extract Enrichment: | Lyophilization |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Sodium phosphate buffer |
