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MB Sample ID: SA183718
Local Sample ID: | MGH270 |
Subject ID: | SU002025 |
Subject Type: | Plant |
Subject Species: | Zea mays |
Taxonomy ID: | 4577 |
Age Or Age Range: | 17 day after germination |
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Sample Preparation:
Sampleprep ID: | SP002031 |
Sampleprep Summary: | Whole samples were ground into fine powder under liquid nitrogen temperature and aliquoted 25 mg for metabolite analysis. The metabolites were extracted by methanol: water: chloroform and 50 µl of the upper aqueous phase was dried down to be derivatized by methoxyamination and trimethylsilylation. |
Processing Storage Conditions: | Room temperature |
Extract Storage: | Room temperature |
Sample Resuspension: | pyridine |
Sample Derivatization: | methoxyamination and trimethylsilylation |
Sample Spiking: | 8.2 ug/ml ribitol |