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MB Sample ID: SA184286
| Local Sample ID: | Healthy24h_9 |
| Subject ID: | SU002035 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 53 ± 15 |
| Weight Or Weight Range: | 116 ± 25 |
| Gender: | Male and female |
| Human Inclusion Criteria: | (i) body mass index (BMI)>35kg/m2 plus GFR 30–60ml/min and proteinuria >1 g/24h or GFR>60ml/min and proteinuria >2.5 g/24h despite receiving maximally tolerated doses of renin-angiotensin-aldosterone system (RAAS) blocker and (ii) BMI>40kg/m2 with a GFR>30ml/min and proteinuria >0.5 g/24h despite receiving maximally tolerated doses of RAAS blocker. For the renal function criterion, eGFR was used. The follow-up time was 24 months. |
| Human Exclusion Criteria: | 1) Patients who had participated or were participating in another clinical trial or had taken an experimental drug in the last 28 days. 2) Patients with renal transplantation and/or chronic replacement therapy (hemodialysis and/or peritoneal dialysis). 3) Subjects with poorly controlled blood pressure (SBP > 170 mmHg or DBP > 110 mmHg). 4) Patients with a history of cardiovascular events in the past six months. 5) Patients treated with immunosuppressants. 6) Subjects with a history of renovascular disease, autoimmune diseases, cancer, drug use, or obstructive uropathy. 6) Patients who did not sign the informed consent. 7) Patients who were pregnant or lactating. eGFR was used to establish the renal function criteria. Patients were monitored for 24 months. |
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Sample Preparation:
| Sampleprep ID: | SP002041 |
| Sampleprep Summary: | 100 µL of each urine sample were mixed in a 100 µL IS (internal standard) mixture with 0.4 mM of methionine sulfone and 2 mM of paracetamol containing 5% of acetonitrile, then, samples were vortexed. Some CKD patients showed an elevated proteinuria and in order to avoid capillary collapse, a Merck Milipore filters (Ultrafiltration Device with Ultracel YM-T membrane, product 4104) were used. Samples mixed with IS mixture were added to filters and centrifuged (2000 g, 60 min, 4 °C). Then, 90 µl of each sample was transferred to a glass vial. Samples were stored at -20°C until analysis. Stability and reproducibility of the system were checked with quality control (QC) samples. Four types of QC samples were prepared combining 60 µl of each sample after filtration: 1. QC from OD and OD BS samples (QC CKD). 2. QC from O and O BS samples (QC Control). 3. QC from OD, OD BS and O and OD BS samples (QC CC). 4. QC from Healthy 1H and Healthy 24H samples (QC Healthy). QC samples were vortex-mixed, transferred to a vial and stored at -20°C until analysis. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | On ice |
