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MB Sample ID: SA184889
| Local Sample ID: | VLC_12hr_3 |
| Subject ID: | SU002045 |
| Subject Type: | Other organism |
| Subject Species: | Microchloropsis |
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Sample Preparation:
| Sampleprep ID: | SP002051 |
| Sampleprep Summary: | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. |
| Processing Storage Conditions: | Described in summary |
| Extraction Method: | Sonication |
| Sample Derivatization: | MSTFA |
| Sample Spiking: | Ribitol (Internal Standard) |
