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MB Sample ID: SA194037

Local Sample ID:	71875_6
Subject ID:	SU002144
Subject Type:	Plant
Subject Species:	Lithospermum officinale
Taxonomy ID:	475907

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Sample Preparation:

Sampleprep ID:	SP002150
Sampleprep Summary:	The lyophilised roots were ground to a fine powder using a ball mill (Fritsch Pulverisette 0, Germany). For each powdered sample, a subsample of 35 mg was placed into microcentrifuge tubes for SMs extraction with 1.5 ml of methanol (LC-MS grade, Honeywell Riedel de Haën, USA) in an ultrasound bath at 10% power for three hours (Bandelin Sonorex Digital 10P, Berlin, Germany) followed by centrifugation for 10 minutes at 12500 rpm (Hermle Z 216 MK, Wehingen, Germany). The supernatants were collected and subjected to Ultra-High-Performance Liquid Chromatography-High Resolution Mass Spectrometry (UHPLC-HRMS) analysis, after filtering with 0.22 μm polytetrafluorethylene (PTFE) filters.

Sampleprep ID:

SP002150

Sampleprep Summary:

The lyophilised roots were ground to a fine powder using a ball mill (Fritsch Pulverisette 0, Germany). For each powdered sample, a subsample of 35 mg was placed into microcentrifuge tubes for SMs extraction with 1.5 ml of methanol (LC-MS grade, Honeywell Riedel de Haën, USA) in an ultrasound bath at 10% power for three hours (Bandelin Sonorex Digital 10P, Berlin, Germany) followed by centrifugation for 10 minutes at 12500 rpm (Hermle Z 216 MK, Wehingen, Germany). The supernatants were collected and subjected to Ultra-High-Performance Liquid Chromatography-High Resolution Mass Spectrometry (UHPLC-HRMS) analysis, after filtering with 0.22 μm polytetrafluorethylene (PTFE) filters.